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Narrative Review

Vol. 2 No. 2 (1996)

Alpha-Fetoprotein (AFP): Solved and Unsolved Problems

  • Garry I. Abelev, Ph.D.
  • Natalia L. Lazarevich
November 1, 2020




  1. Bergstrand CG, Czar B. Demonstration of a new protein fraction in serum from the human fetus. Scandinavian Journal of Clinical Laboratory and Investigations 8: 174-179; 1956.
  2. Abelev GI. Alpha-fetoprotein: the genesis. Oncodevelopmental Biology and Medicine 4: 371-381; 1983.
  3. Abelev GI, Perova S, Khramkova NI, et al. Production of embryonal alpha-globulin by the transplantable mouse hepatomas. Transplantation 1: 174-180; 1963.
  4. Tatarinov YS. Presence of embryonal alpha-globulin in the serum of patient with primary hepatocellular carcinoma. Voprosy Medicinskoy Chimii 10: 90-91; 1964.
  5. Abelev GI. Alpha-fetoprotein in ontogenesis and its association with malignant tumors. Advances in Cancer Research 14: 295-358; 1971.
  6. Abelev GI. The origin of the cancer immunodiagnosis. In: Mazumdar PMH, ed. Immunology 1930-1980. Essays on the History of Immunology. Toronto: Wall & Tompson; 1989: 275-290.
  7. Abelev GI, Assecritova IV, Kraevsky NA, et al. Embryonal serum alpha-globulin in cancer patients: diagnostic value. International Journal of Cancer 2: 551-558; 1967.
  8. Masopust J, Kithier R, Radl I, et al. Occurrence of fetoprotein in patients with neoplasms and non-neoplastic disease. International Journal of Cancer 3:364-370; 1968.
  9. Abelev GI. Alpha-Fetoprotein as a marker of embryo-specific differentiations in normal and tumor tissues. Transplantation Reviews 20: 3-37; 1974.
  10. Ruoslahti E, Seppala M. Studies of carcino-fetal proteins. III. Development of radio-immunoassay for alpha-fetoprotein. Demonstration of alpha-fetoprotein in serum of healthy human adults. International Journal of Cancer 8: 374-383, 1971.
  11. Brock D, Sutcliffe R. Alpha-fetoprotein in antenatal diagnosis of anencephaly and spina bifida. Lancet ii:197; 1972.
  12. Milunsky A, Alper E,. Results and benefits of a maternal serum alpha-fetoprotein screening program. Journal of American Medical Association 252: 1438-1442; 1984.
  13. Norgaard-Pedersen B, Larsen SO, Anders J, et al. Maternal serum markers in screening for Down syndrome. Clinical Genetics 36: 1-9; 1989.
  14. Masseyeff R. Human alpha-feto-protein (Review). Pathologie Biologie 20: 703-727; 1972.
  15. Abelev GI, Elgort DA. Alpha-fetoprotein. In: Holland J, Frei E, eds. Cancer Medicine, 2nd edition. New York: Lea & Febiger 1982: 1089-1099.
  16. Abelev GI. Alpha-fetoprotein: 25 years of study. Tumor Biology 10: 63-74; 1989.
  17. Abelev GI. Antigenic structure of chemically-induced hepatomas. Progress in Experimental Tumor Research 7: 104-157; 1965.
  18. Deutsch HF. Chemistry and biology of alpha-fetoprotein. Advances in Cancer Research 56: 253-312; 1991.
  19. Mizejewski GI. The filogeny of alpha-fetoprotein in vertebrates: survey of biochemical and physiological data. Critical Reviews in Eucariotic Gene Expression 5: 281-316; 1995.
  20. Ruoslahti E, Seppala M. Alpha-Fetoprotein in cancer and fetal development. Advances in Cancer Research 29: 275-346; 1979.
  21. Christiansen M, Yazova AK, Karamova ER, et al. Epitopes of human alpha-fetoprotein. Journal of Tumor Marker Oncology 9: 57-68; 1994.
  22. Kitatawa H, Ohkouchi E, Jan-Ichi H, et al. Monoclonal antibodies with fine specifities distinguishing alphafetoprotein of hepatoma and yolk sac tumor origin. International Japanese Cancer Research 77: 1012-1017; 1986.
  23. Smith C, Kelleher P. Alpha-fetoprotein molecular heterogeneity. Physiologic correlation with normal growth, carcinogenesis and tumor growth. Biochemica and Biophysica Acta 605: 1-32; 1980.
  24. Breborowich J. Microheterogeneity of human alphafetoprotein. Tumor Biology 9: 3-14; 1988.
  25. Taketa K. Alpha-Fetoprotein: reevaluation in hepatology. Hepatology 12: 1420-1432; 1990.
  26. Yazova AK, Yakimenko EF, Goussev AI, et al. Differences in epitope structure of human AFP revealed by monoclonal antibodies. Abstracts of XXIV ISOBM Meeting, San-Diego, 1996 (In press).
  27. Parmelee M, Evenson M, Deutsch H. The presence of fatty acids in human alpha-fetoprotein. Journal of Biological Chemistry 253: 2114-2119; 1978.
  28. Uriel J, Laborda J, Naval J, et al. AFP receptors in malignant cells (an overview). In: Mizejewski GI, Jacobson HI, eds. Biological Activities of Alpha1-Fetoprotein, vol 2. Boca Raton, Florida: CRC Press; 1989: 103-118.
  29. Hsia JC, Wong LT, Trimble CE, et al. In: Mizejewski GI, Jacobson HI, eds. Biological Activities of Alpha1-Fetoprotein, vol 1. Boca Raton, Florida: CRC Press; 1987: 189-204.
  30. Moro R. The AFP receptor - a widespread oncofetal antigen. In: Mizeyewski GI, Jacobson HI, eds. Biological Activities of Alpha1-Fetoprotein, vol 2. Boca Raton, Florida: CRC Press; 1989: 119-128.
  31. Deutsch H. The uptake of adriamycyn-arachidonic acid complexes by human tumor cells in the presence of alpha-fetoprotein. Journal of Tumor Marker Oncology 9: 11-14; 1994.
  32. Nunez EA, Engelman F, Benassayag C, et al. Identification et purification preliminaire de la foetoproteine liant les oestrogenes dan le serumde rats nourveau-nes. Comptes Rendue de'l Academie de Sciences (Paris) 273: 831-834; 1971.
  33. Nunez EA, Benassayag C, Christeff N, et al. Estrogen and fatty acid binding properties of murine AFP: a guide to explain some biological activities of this protein. In: Mizeyewski GI, Jacobson HI, eds. Biological Activities of Alpha1-Fetoprotein, vol 2. Boca Raton, Florida: CRC Press; 1989: 3-18.
  34. Mizeyewski GI, Stanton BR, Jacobson HI. AFP modification of biological response in estrogen-sensitive tissues. Use of in vivo and in vitro models. In: Mizeyewski GI, Jacobson HI, eds. Biological Activities of Alpha1-Fetoprotein, vol 2. Boca Raton, Florida: CRC Press; 1989: 59-74.
  35. Mizejewski GI, Jacobson HI, eds. Biological Activities of Alpha1-Fetoprotein. Boca Raton, Florida: CRC Press; vol 1, 1987; vol 2, 1989.
  36. Lester EP, Miller JB, Baron JM, et al. Inhibition of human lymphocyte transformation by human alpha-fetoprotein (HAFP): studies on the mode of HAFP action and the role of HAFP polymorphism. Immunology 34: 189-194; 1976.
  37. Lu CY, Changelian PS, Unanue ER. Alpha-fetoprotein inhibits macrophage expression of Ia antigen. Journal of Immunology 132: 1722-1727; 1984.
  38. Gitlin D, Boesman M. Sites of serum alpha-fetoprotein synthesis in the human and rat. Journal of Clinical Investigations 46: 1010-1016; 1967.
  39. Gitlin D, Pericelli A, Gitlin C. Synthesis of alpha-fetoprotein by liver, yolk sac and gastrointestinal tract of the human concepts. Cancer Research 32: 979-987; 1972.
  40. Dziadek M. Modulation of alpha-fetoprotein synthesis in the early post-implantation embryo. Journal of Embryology and Experimental Morphology 46: 135-146; 1978.
  41. Gleiberman AS, Abelev GI. Cell position and cell interactions in expression of fetal phenotype of hepatocyte. International Review of Cytology 95: 229-266; 1985.
  42. Abelev GI. Study of the regulation of alpha-fetoprotein synthesis in ontogenesis and carcinogenesis. In: Skulachev VP, ed. Soviet Science Review (section D) Biological Reviews. New York: Harwood Acad Publ.: 1: 371-397; 1980.
  43. Baranov VN, Engelhardt NV. Immunoperoxidase technique in electron microscopy: theoretical and practical aspects and use of localization of alpha-fetoprotein synthesis. In: Blokhin NN, Trapeznikov NN, eds. Soviet Medical Reviews (Section F) Oncology. New York: Harwood Acad Publ; 1: 119-163; 1987.
  44. Gleiberman AS, Kudryavtseva EI, Sharovskaya YY, et al. The synthesis of alpha-fetoprotein in hepatocytes is coordinately regulated with cell-cell and cell-matrix interactions. Molecular Biology and Medicine 6: 95-107; 1989.
  45. Harper ME, Dugaiczyk A. Linkage of the evolutionary related serum albumin and alpha-fetoprotein genes within q11-22 of human chromosome 4. American Journal of Human Genetics 35: 565-572; 1983.
  46. Belanger L, Sylvie R, Allard D. New albumin gene 3' adjacent to the alpha-fetoprotein locus. Journal of Biological Chemistry 269: 5481-5484; 1994.
  47. Gibbs PEM, Zielinski R, Boyd C, et al. Structure, polymorphism, and novel repeat DNA elements revealed by a complete sequence of the human alpha-fetoprotein gene. Biochemistry 26: 1332-1341; 1987.
  48. Godbout R, Ingram R, Tilghman SM. Fine-structure mapping of the three mouse alpha-fetoprotein gene enhancers. Molecular and Cellular Biology 8: 1169-1178; 1988.
  49. Guertin M, La Rue H, Bernier D, et al. Enhancer and promoter elements directing activation and glucocorticoid repression of the alpha-fetoprotein gene in hepatocytes. Molecular and Cellular Biology 8: 1398-1407; 1988.
  50. Wen P, Group ER, Bruzard G, et al. Enhancer, repressor and promoter specificities combine to regulate the rat alpha-fetoprotein gene. DNA and Cell Biology 10: 525-536; 1991.
  51. Vacher J, Tilghman SM. Dominant negative regulation of the mouse alpha-fetoprotein gene in adult liver. Science 250: 1732-1735; 1990.
  52. Ramesh TM, Ellis AW, Spear BT. Individual mouse alpha-fetoprotein enhancer elements exhibit different patterns of tissue-specific and hepatic position-dependent activities. Molecular and Cellular Biology 15: 4947-4955; 1995.
  53. Bernier D, Thomassin H, Allard D, et al. Functional analysis of developmentally regulated chromatin-hypersensitive domains carrying the alpha-fetoprotein intergenic enhancer. Molecular and Cellular Biology 13: 1619-1633; 1993.
  54. Wen P, Locker J. A novel hepatocyte transcription factor that binds the alpha-fetoprotein promoter-linked coupling element. Molecular and Cellular Biology 14: 6616-6626; 1994.
  55. Tronche F, Yaniv M. HNF-1, a homeoprotein member of the hepatic transcription regulatory network. BioEssays, 14: 579-587; 1992.
  56. Belayew A, Tilghman SM. Genetic analysis of alpha-fetoprotein synthesis in the mouse. Molecular and Cellular Biology 2: 1427-1435; 1982.
  57. Vacher J, Camper SA, Krumlauf R, et al. raf regulates the postnatal repression of the mouse alpha-fetoprotein gene at the posttranscriptional level. Molecular and Cellular Biology 12: 856-864; 1992.
  58. Spear BT. Mouse alpha-fetoprotein gene 5' regulatory elements are required for postnatal regulation by raf and Rif. Molecular and Cellular Biology 14: 6497- 6505; 1994.
  59. Yasuda H, Mizuno A, Tamaoki T, et al. ATBF1, a multiple-homeodomain zinc finger protein, selectively down-regulates AT-rich elements of the human alpha-fetoprotein gene. Molecular and Cellular Biology 14: 1395-1401; 1994.
  60. Engelhardt NV, Poltoranina VS, Yazova AK. Localization of alpha-fetoprotein in transplantable murine teratocarcinomas. International Journal of Cancer 11;448-459; 1973.
  61. Teilum G. Special tumors of ovary and testis, II edition. Copenhagen: Munsgaard; 1976.
  62. Lightner DJ, Longe P. Testicular tumors. In: Herberman RB, Mercer DW, eds. Immunodiagnosis of Cancer, II edition. New York: Marcel Dekker; 1990: 397-401.
  63. Eraizer TL, Khamzina LS. Phenotypic variability of cultured rat hepatoma cell population in respect to alpha-fetoprotein synthesis. International Journal of Cancer 42: 633-637; 1988.
  64. Kutagawa T, Yokochi T, Sugano H. Alpha-Fetoprotein and hepatocarcinogenesis in rats fed 3'-methyl-4- (dimethylamino)azobenzene and N-2-fluorenyl-acetate. International Journal of Cancer 10: 368-381; 1972.
  65. Sell S. Is there a liver stem cell? Cancer Research 50: 3811-3815; 1990.
  66. Sell S, Hunt J, Knoll B, et al. Cellular events during hepatocarcinogenesis in rats and the question of premalignancy. Advances in Cancer Research 48: 37-111; 1987.
  67. Wu AHB, Sell S. Markers of hepatocellular carcinoma. In: Herberman RB, Mercer DW, eds. Immunodiagnosis of Cancer, II edition. New York: Marcel Dekker; 1990: 403-422.
  68. Burditte LJ, Johnson MM, Johnson PI, et al. Detection of hepatocellular carcinoma-specific alpha- fetoprotein by isoelectric focusing. Cancer 74, 25-29; 1994.
  69. Masseyeff R. Alpha-fetoprotein -- use in screening. In: Herberman RB, McIntire KR, eds. Immunodiagnosis of Cancer, I edition. New York: Marcel Dekker; 1979: 117-130.
  70. Xu K-L. Large screening for hepatocellular carcinoma in China. In: Fishman WH, ed. Oncodevelopmental Markers. New York: Academic Press; 1983: 395-408.
  71. Lehmann F-G. Prognostic significance of alpha-1-fetoprotein in liver cirrhosis. Five-years perspective study. In: Fishman WH, Sell S, eds. Oncodevelopmental Gene Expression. New York: Academic Press; 1976: 407-416.
  72. McMahon B, London T. Workshop on screening on hepatocellular carcinoma. Journal of National Cancer Institute 83: 916-920; 1991.
  73. Abelev GI. Alpha-fetoprotein biology. In: Skulachev VP, ed. Soviet Science Review (section D) Physicochemical Biology. New York: Harwood Acad Publ; 11: 85-109; 1993.
  74. Christiansen M, Ishiguro T, Hogdall C, et al. Alpha-fetoprotein. In: Ballesta AM, Torre G, Bombardieri E, et al, eds. Up Dating of Tumor Markers in Tissues and in Biological Fluids. Torino: Edizoni Minerva Medica; 1993: 245-269.


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