Vol. 11 No. 2 (2008)
Research Article

Effects of Repeatedly Heated Palm Oil on Serum Lipid Profile, Lipid Peroxidation and Homocysteine Levels in a Post-Menopausal Rat Model

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  • Siti Khadijah Adam,
  • Ima Nirwana Soelaiman,
  • Nor Aini Umar,
  • Norhayati Mokhtar,
  • Norazlina Mohamed,
  • Kamsiah Jaarin
DOI: https://doi.org/10.26443/mjm.v11i2.566

Published 2020-12-01

Keywords

  • palm oil,
  • heated oil,
  • menopause,
  • atherosclerosis,
  • lipid peroxidation

How to Cite

1.
Adam SK, Soelaiman IN, Umar NA, Mokhtar N, Mohamed N, Jaarin K. Effects of Repeatedly Heated Palm Oil on Serum Lipid Profile, Lipid Peroxidation and Homocysteine Levels in a Post-Menopausal Rat Model. McGill J Med [Internet]. 2020 Dec. 1 [cited 2025 Oct. 31];11(2). Available from: https://mjm.mcgill.ca/article/view/566
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Abstract

Oxidized unsaturated fatty acids may contribute to the pathogenesis of atherosclerosis. In the present study, we examined the effects of heated palm oil mixed with 2% cholesterol diet on serum lipid profile, homocysteine and thiobarbituric acid reactive substances (TBARS) levels in estrogen-deficient rats. Twenty-four female Sprague Dawley rats were ovariectomized and then were divided equally into four groups. The control group was given 2% cholesterol diet only throughout the study period. The three treatment groups received 2% cholesterol diet fortified with fresh, once-heated or five-times-heated palm oil,
respectively. Serum TBARS, lipid profile and homocysteine levels were measured prior to ovariectomy and at the end of four months of the study. Five-times-heated palm oil caused a significant increase in TBARS and total cholesterol (TC) compared to control (F = 22.529, p < 0.05). There was a significant increase in serum homocysteine in the control as well as five-times heated palm oil group compared to fresh and once-heated palm oil groups (F = 4.432, p < 0.05). The findings suggest that repeatedly heated palm oil increase lipid peroxidation and TC. Ovariectomy increases the development of atherosclerosis as seen in this study. Feeding with fresh and once-heated palm oil does not cause any deleterious effect but repeatedly heated oil may be harmful because it causes oxidative damage thereby predisposing to atherosclerosis.

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References

  1. Rossouw JE. Hormones, genetic factors and gender differences in cardiovascular disease. Cardiovasc Res. 2002; 53(3): 550-557.
  2. Baker L, Meldrum KK, Wang M, et al. The role of estrogen in cardiovascular disease. J Surg Res. 2003; 115(2): 325-344.
  3. Nabulsi AA, Folsom AR, White A, et al. Association of hormone-replacement therapy with various cardiovascular risk factors in postmenopausal women. The Atherosclerosis Risk in Communities Study Investigators. N Eng J Med. 1993; 328(15): 1069-1075.
  4. Ayres S, Tang M, Ravi Subbiah MT. Estradiol-17β as an antioxidant: some distinct features when compared with common fat-soluble antioxidants. J Lab Clin Med. 1996; 128(4): 367-375.
  5. Badeau M, Adlercreutz H, Kaihovaara P, Tikkanen MJ. Estrogen A-ring structure and antioxidative effect on lipoproteins. J Steroid Biochem Mol Biol. 2005; 96(3-4): 271-278.
  6. Osterud B, Bjorklid E. Role of monocytes in atherogenesis. Physiol Rev. 2003; 83(4): 1069-1112.
  7. Plutzky J, Libby P. Pathophysiology of atherosclerotic heart disease. In: Tonkin AM, editor. Atherosclerosis and heart disease. London, UK: Martin Dunitz; 2003. p. 1-12.
  8. Steinberg D, Witztum JL. Lipoproteins and atherogenesis. Current concepts. JAMA. 1990; 264(23): 3047-3052.
  9. Su SJ, Huang LW, Pai LS, Liu HW, Chang KL. Homocysteine at pathophysiologic concentrations activates human monocyte and induces cytokine expression and inhibits macrophage migration inhibitory factor expression. Nutrition 2005; 21(10): 994-1002.
  10. Welch GN, Loscalzo J. Homocysteine and atherothrombosis. N Engl J Med. 1998; 338(15): 1042-1050
  11. Olszewski AJ, McCully KS. Homocysteine metabolism and the oxidative modification of proteins and lipids. Free Radic Biol Med. 1993; 14(6): 683-693.
  12. Dobarganes C, Márquez-Ruiz G. Oxidized fats in foods. Curr Opin Clin Nutr Metab Care 2003; 6(2): 157-163.
  13. Esterbauer H, Schaur RJ, Zollner H. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic Biol Med. 1991; 11(1): 81-128.
  14. Owu DU, Osim EE, Ebong PE. Serum liver enzymes profile of Wistar rats following chronic consumption of fresh or oxidized palm oil diets. Acta Trop. 1998; 69(1): 65-73.
  15. Izaki Y, Yoshikawa S, Uchiyama M. Effect of ingestion thermally oxidized frying oil on peroxidative criteria in rats. Lipids 1984; 19(5): 324-331.
  16. Staprans I, Rapp JH, Pan XM, Hardman DA, Feingold KR. Oxidized lipids in the diet accelerate the development of fatty streaks in cholesterol-fed rabbits. Arterioscl Throm Vasc Biol. 1996; 16(4): 533-538.
  17. Benedetti PC, D’Aquino M, Di Felice M, Gentili V, Tagliamonte B, Tomassi G. Effects of a fraction of thermally oxidized soy bean oil on growing rats. Nutr Rep Int. 1987; 36(2): 387-401.
  18. Ledwozyw A, Michalak J, Stepien A, Kadziolka A. The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. Clin Chim Acta 1986; 155(3): 275-283.
  19. Lowry OH, Rosebrugh NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1): 265-75.20. Maxwell SRJ, Lip GYH. Free radicals and antioxidants in cardiovascular disease. Br J Clin Pharmacol 1997; 44(4): 307-317.
  20. Kamsiah J, Nafeeza MI, Ngang AC. Suppression of atherogenesis in cholesterol-fed rabbits treated with nicardipine. Asia Pac J Pharm. 1994; 9(3): 183-187.
  21. Nwanguma BC, Achebe AC, Ezeanyika LUS, Eze LC. Toxicity of oxidized fats II: tissue levels of lipid peroxides in rats fed a thermally oxidized corn oil diet. Food Chem Toxicol. 1999; 37(4): 413-416.
  22. Cottrell RC. Introduction: nutritional aspects of palm oil. Am J Clin Nutr. 1991; 53(4 Suppl): 989S-1009S.
  23. Hageman G, Verhagen H, Schutte B, Kleinjans J. Biological effects of short-term feeding to rats of repeatedly used deepfrying fats in relation to fat mutagen content. Food Chem Toxicol. 1991; 29(10): 689-698.
  24. Hur SJ, Du M, Nam K, Williamson M, Ahn DU. Effect of dietary fats on blood cholesterol and lipid and the development of atherosclerosis in rabbits. Nutr Res. 2005; 25(10): 925-935.
  25. Adam SK, Sulaiman NA, Md Top AG, Jaarin K. Heating reducesvitamin E content in palm and soy oils. Malays J Biochem Molec Biol. 2007; 15(2): 76-79.
  26. Kamsiah J, Norhayati M, Norzana G, Nor Aini U, Ima-Nirwana S. Effects of heated vegetable oils on serum lipids and aorta of ovariectomized rats. Pak J Nutr. 2006; 5(1): 19-29.
  27. Rueda-Clausen CF, Silva FA, Lindarte MA, et al. Olive, soybean and palm oils intake have a similar acute detrimental effect over the endotelial function in healthy young subjects. Nutr Metab Cardiovas Dis. 2007; 17(1): 50-57.
  28. Adam SK, Das S, Soelaiman IN, Umar NA, Jaarin K. Consumption of repeatedly heated soy oil increases the serum parameters related to atherosclerosis in ovariectomised rats. Tohoku J Exp Med. 2008; 215(3): 219-226.
  29. Witztum JL, Steinberg D. Role of oxidized low density lipoprotein in atherogenesis. J Clin Invest. 1991; 88(6): 1785-1792.
  30. Lapointe A, Couillard C, Lemieux S. Effects of dietary factors on oxidation of low-density lipoprotein particles. J Nutr Biochem. 2006; 17(10): 645-658.
  31. Shuid AN, Chuan LH, Mohamed N, Jaarin K, Fong YS,